9th International Conference on Sustainable Solid Waste Management

Mixotrophic and heterotrophic cultivation of Auxenochlorella protothecoides in chicken manure extract supplemented with sodium acetate: effect of substrate C/N ratio on biochemical composition.

Introduction

Recovery of nutrient and energy from agro-industrial wastes is gaining attention in the context of circular economy. Substantial amounts of chicken manure (CM) are annually generated in the frame of livestock production (Yin et al., 2021). Microalgae are advantageous microorganisms which can grow in various substrates under mixotrophic or heterotrophic conditions. Nitrogen source and carbon to nitrogen (C/N) ratio in the culture medium influence the biomass growth and biochemical composition (Chalima et al., 2017). In this study, Auxenochlorella protothecoides was cultivated in a protein-rich extract of CM supplemented with sodium acetate in order to simulate a volatile fatty acid (VFA) rich substrate after CM acetogenetic fermentation. The effect of different C/N ratios and light conditions (heterotrophy and mixotrophy) on the biomass productivity and biochemical composition as a potential feed supplement was examined.